磁性Fe3O4-羧甲基壳聚糖纳米粒的制备及光热性能研究

沈松,吴琳,戚雪勇,戈延茹*,金一*

中国药学杂志 ›› 2014, Vol. 49 ›› Issue (16) : 1432-1436.

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中国药学杂志 ›› 2014, Vol. 49 ›› Issue (16) : 1432-1436. DOI: 10.11669/cpj.2014.16.015
论著

磁性Fe3O4-羧甲基壳聚糖纳米粒的制备及光热性能研究

  • 沈松12,吴琳3,戚雪勇1,戈延茹1*,金一2*
作者信息 +

Preparation and Photothermal Effect Evaluation of Magnetic Fe3O4-CMCTS Nanoparticles

  • SHEN Song1, 2, WU Lin3, QI Xue-yong1, GE Yan-ru1*, JIN Yi2*
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摘要

目的 制备具有良好生物相容性的磁性纳米粒,并对其光热性能、细胞毒性及体外光热治疗效果进行研究。方法 采用水热法合成Fe3O4,分别利用羧甲基壳聚糖(CMCTS)及壳聚糖(chitosan)对其进行功能化修饰,采用TEM、TGA等手段对制备的载体进行表征,考察纳米粒的稳定性及光热性能,同时采用MTT方法研究载体的细胞毒性、评价其光热治疗效果。结果 所制备的Fe3O4粒径均一、分散良好,CMCTS修饰的Fe3O4具有良好的稳定性;Fe3O4-CMCTS纳米粒无明显毒性,Fe3O4-Chitosan由于稳定性原因显示一定的细胞毒性;光热治疗结果显示,浓度为60 μg·mL-1的2种载体在808 nm激光(2 W·cm-2)照射5 min时,可杀伤90%以上的细胞。结论 所制备的磁性Fe3O4-CMCTS纳米粒具有良好的稳定性、较低的细胞毒性及优良的光热效果,可以作为新型光热材料深入研究。

Abstract

OBJECTIVE To prepare magnetic nanoparticles with good biocompatiblity and evaluate the photothermal effect, cytotoxicity and photothermal abltion of cancer cells in vitro. METHODS The magnetic nanoparticles were sythesized using a modified solvothermal reaction and then modified with O-carboxymethyl chitosan (CMCTS) and chitosan respectively. The prepared magneic particles were characterized with TEM and TGA. Then the stability and photothermal effect were evaluated. MTT method was ued to study the cytotoxicity and cancer therapeutic effect of the magnetic nanoparticles. RESULTS The obtained Fe3O4nanoparticles were uniform and well dispersed.After modification with CMCTS, Fe3O4 showed good stability and photothermal effect. No obvious cytotoxicity was observed in Fe3O4-CMCTS treated cells. Fe3O4-chitosan exhibited some cytotoxicity for the poor stability. After being treated with Fe3O4-CMCTS or Fe3O4-Chitosan at the concentration of 60 μg·mL-1and exposed to 808 nm laser for 5 min at a low power density of 2 W·cm-2, over 90% of KB cells were destructed.CONCLUSION The obtained Fe3O4-CMCTS magnetic nanoparticles had favourable stability, low cytotoxicity and excellent photothermal effect, and should be studied further as a novel photothermal agent.

关键词

光热治疗 / 磁性四氧化三铁 / 细胞毒性 / 多功能纳米粒

Key words

photothermal therapy / magnetic Fe3O4 / cytotoxicity / multifunctional nanoparticle

引用本文

导出引用
沈松,吴琳,戚雪勇,戈延茹*,金一*. 磁性Fe3O4-羧甲基壳聚糖纳米粒的制备及光热性能研究[J]. 中国药学杂志, 2014, 49(16): 1432-1436 https://doi.org/10.11669/cpj.2014.16.015
SHEN Song, , WU Lin, QI Xue-yong, GE Yan-ru*, JIN Yi*. Preparation and Photothermal Effect Evaluation of Magnetic Fe3O4-CMCTS Nanoparticles[J]. Chinese Pharmaceutical Journal, 2014, 49(16): 1432-1436 https://doi.org/10.11669/cpj.2014.16.015
中图分类号: R944   

参考文献

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基金

国家自然科学基金资助项目(81172999)
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